Bacillus amyloliquefaciens uses mixed sugar to synthesize γ-PGA

2018 5th International Conference on Advanced Composite Materials and Manufacturing Engineering

Sui, Ming^1,2

Key Laboratory of Leather Chemistry and Engineering, Ministry of Education, College of Light Industry Textile and Food Engineering, Sichuan University, Chengdu

610065, China^1

Department of Wine and Food Engineering, Si Chuan Technology and Business College, Du jiang-yan, Sichuan

611800, China^2

关键词: Application prospect;    Bacillus amyloliquefaciens;    Carbon and nitrogen;    Culture conditions;    Fermentation medium;    Poly-gamma-glutamic acid;    Polyglutamic acids;    Water solubilities;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/394/2/022069/pdf
DOI  :  10.1088/1757-899X/394/2/022069

来源: IOP

【 摘 要 】

Poly-γ-glutamic acid (γ-PGA) is an anionic polymer synthesized by microorganisms. It has very strong water solubility, edible property and biocompatibility. It is non-toxic to humans and the environment. It has wide application prospect in agriculture, food industry, pharmacy and environment. However, its fermentation cost is high, and its fermentation medium needs a large amount of carbon and nitrogen sources such as glucose, citric acid and glutamic acid, while lignocellulose As one of the most abundant biomass resources on the planet, it can hydrolyze glucose and xylose. Therefore, it is possible to select suitable fermentation strains and utilize lignocellulose to optimize culture conditions to improve the efficiency of γ-PGA synthesis. In this paper, a self-screening bacillus amyloliquefaciensC1 was studied and found that the bacteria can use xylose as a carbon source for growth and metabolism. Using bacillus amyloliquefaciensFZB42 as a control to study in depth, two strains of bacteria were cultured in M9 containing 10g/L xylose, and when the culture time reached 44h, the strain C1 had almost completely consumed 10g/L of xylose, while the strain FZB42 Only consumed 2g/L of xylose. In response to this phenomenon, we will be Cl bacteria can effectively use the nature of xylose used in polyglutamic acid fermentation experiments, 60g/L of xylose as a carbon source can produce polyglutamic acid 14.52g/L, while the same concentration Of glucose as carbon source, can produce polyglutamic acid 9.64g/L.