Optimization of biosurfactant production from Vibrio sp. BSM-30 isolated in tropical waters

International Conference on Energy Engineering and Environmental Protection 2016

能源学;生态环境科学

Su, Zengjian^1,2 ; Li, Min^3 ; Zhang, Yuxiu^1

China University of Mining and Technology (Beijng), School of Chemical and Environmental Engineering, Beijng

100083, China^1

Environment and Plant Protection College, Hainan University, Haikou, Hainan Province

570228, China^2

CATAS, Environment and Plant Protection Research Institute, Haikou, Hainan Province

571737, China^3

关键词: Bio surfactant;    Biosurfactant production;    Carbon source;    Culture conditions;    Nitrogen sources;    Shaking speed;    Soluble starch;    Tropical waters;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/52/1/012069/pdf
DOI  :  10.1088/1742-6596/52/1/012069

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The strain BSM-30 (Vibrio sp.), isolated from Chinese tropical waters, could be a biosurfactant producing bacteria according with results obtained by the oil spreading method. The culture conditions for biosurfactant production were tested respectively such as inoculation (2%,6%,10%,14% as setting), shaking speed(120 r/min,150 r/min,180 r/min as setting), temperature (25°C,30°C,35°C as setting), pH (7,8,9 as setting), salinity (1.5%, 2.5%, 3.0%, 4.5%, 5.5% as setting), which results showed that the best culture conditions for BS production were 10% inoculation quantity, 180 r/min, 25°C, pH 8, and 3.5% salinity. The optimization of carbon sources (20g/ of glucose, 20g/L of starch, 20g/L of paraffin oil 20g/L of diesel, 20g/L of oil as setting) and nitrogen sources (6g/L of NaNO3,7.1g/L of KNO3,5.6g/L of NH4NO3,9.3g/L of (NH4)2SO4, 4.2g/L of CO(NH2)2as setting) were also tested, which results showed that the best nitrogen source and carbon source were (NH4)2SO4and soluble starch.