Biosurfactant production by Rhodococcus erythropolis and its application to oil removal

Brazilian Journal of Microbiology

Graziela Jardim Pacheco¹  Elisa Mara Prioli Ciapina¹  Edelvio De Barros Gomes¹  Nei Pereira Junior¹ 

[1] ,Universidade Federal do Rio de Janeiro Faculdade de Química Centro de TecnologiaRio de Janeiro RJ ,Brasil

关键词: Biosurfactant production;    oil removal;    Rhodococcus erythropolis;   

DOI  :  10.1590/S1517-83822010000300019

来源: SciELO

【 摘 要 】

The influence of different nutrients on biosurfactant production by Rhodococcus erythropolis was investigated. Increasing the concentration of phosphate buffer from 30 up through 150 mmol/L stimulated an increase in biosurfactant production, which reached a maximum concentration of 285 mg/L in shaken flasks. Statistical analysis showed that glycerol, NaNO3,MgSO4 and yeast extract had significant effects on production. The results were confirmed in a batchwise bioreactor, and semi-growth-associated production was detected. Reduction in the surface tension, which indicates the presence of biosurfactant, reached a value of 38 mN/m at the end of 35 hours. Use of the produced biosurfactant for washing crude oil-contaminated soil showed that 2 and 4 times the critical micellar concentration (CMC) were able to remove 97 and 99% of the oil, respectively, after 1 month of impregnation.

【 授权许可】

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