Optimization Production of Biosurfactant by Pseudomonas putida Using Crude Palm Oil (CPO) as Substrate

International Conference on Green and Renewable Energy Resources 2016

能源学;生态环境科学

Suryanti, V.^1 ; Handayani, D.S.^1 ; Masykur, A.^1 ; Lindasari^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta

57126, Indonesia^1

关键词: Bio surfactant;    Biosurfactant production;    Crude palm oil;    Culture medium;    Emulsification index;    Fermentation time;    Incubation time;    Pseudomonas putida;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/75/1/012013/pdf
DOI  :  10.1088/1755-1315/75/1/012013

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The production of biosurfactant by Pseudomonas putida has been studied. P. putida FNCC 0071 was grown in the nutrient broth medium supplemented with NaCl and crude palm oil (CPO). The effect of CPO concentration and fermentation time on the biosurfactant production were evaluated. The biosurfactant production was evaluated every 24 h for 10 days by optical density, surface tension and emulsification index. The best culture medium was found to be medium containing 5% v/v of CPO with 5 days of incubation time. The biosurfactant was identified as rhamnolipids.