Physicochemical Properties of Biosurfactant Produced by Pseudomonas fluorescens Grown on Whey Tofu

International Conference on Advanced Materials for Better Future 2016

Suryanti, V.^1 ; Handayani, D.S.^1 ; Marliyana, S.D.^1 ; Suratmi, S.^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Sebelas Maret University, Jl. Ir. Sutami 36A, Surakarta

57126, Indonesia^1

关键词: Bio surfactant;    Commercial surfactants;    Critical micelle concentration (cmc);    Emulsion stability;    Physicochemical property;    Pseudomonas fluorescens;    Triton X-100;    Water in oil emulsions;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/176/1/012003/pdf
DOI  :  10.1088/1757-899X/176/1/012003

来源: IOP

【 摘 要 】

The research aims to examine the physicochemical properties of biosurfactant produced by Pseudomonas fluorescens. Biosurfactant was produced in whey tofu media containing 8 g/L nutrient broth and 5 g/L NaCl which was fermented for 2 days at room temperature. Biosurfactant was identified as rhamnolipids which had critical micelle concentration (CMC) value of 638 mg/L and surface tension of 54 mN/m. The biosurfactant had water in oil (w/o) emulsion type. The biosurfactant was able to decrease the interfacial tension more than 40% for emulsion of water with hexane, pentane, benzene, lubricants or kerosene. The stable emulsions were reached up to 30 days with the E24 value of about 50% when paraffin, toluene, lubricants or palm oil was used as an immiscible compound. Commercial surfactants, such as Triton X-100 and Tween-80 were investigated to compare their emulsification activities and emulsion stabilities with the produced biosurfactant.