Preliminary study : optimization of pH and salinity for biosurfactant production from Pseudomonas aeruginosa in diesel fuel and crude oil medium

3rd International Seminar On Sciences "Sciences On Precision And Sustainable Agriculture"

Ikhwani, A.Z.N.^2 ; Nurlaila, H.S.^2 ; Ferdinand, F.D.K.^2 ; Fachria, R.^2 ; Hasan, A.E.Z.^1 ; Yani, M.^2 ; Setyawati, I.^1 ; Suryani^1

Biochemistry Department, Mathematics and Natural Sciences Faculty, Bogor Agricultural University, Dramaga-Bogor, Indonesia^1

Agroindustrial Technology Department, Agricultural Technology Faculty, Bogor Agricultural University, Dramaga-Bogor, Indonesia^2

关键词: Bio surfactant;    Biosurfactant production;    Carbon source;    Environmental factors;    Mineral salts;    Pseudomonas aeruginosa;    Secondary metabolites;    Surface-active compounds;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/58/1/012056/pdf
DOI  :  10.1088/1755-1315/58/1/012056

来源: IOP

【 摘 要 】

Biosurfactant is secondary metabolite surface active compound produced by microorganisms which is nontoxic and eco-friendly. Microorganism producing biosurfactant that is quite potential to use in many applications is from Pseudomonas aeruginosa strains. Good quality of biosurfactant production from microbes is supported by the suitable nutrients and environmental factors. The aim of this research was to obtain preliminary o data upon the optimum pH and salinity for the production of biosurfactant from Pseudomonas aeruginosa ATCC 15442 in diesel fuel and crude oil medium. P. aeruginosa ATCC 15442 cultured in diesel fuel and crude oil as carbon source showed biosurfactant activity. P.aeruginosa-derived biosurfactant was capable to form stable emulsion for 24 hours (EI24) in hydrocarbons n-hexane solutions. The particular biosurfactant showed EI24highest value at pH 7 (31.02%) and 1% NaCl (24.00%) when P. aeruginosa was grown in 10% diesel fuel medium in mineral salt solution. As for the media crude oil, the highest EI24value was at pH 6 (52.16%) and 1% NaCl (33.30%).