Harvesting Chaetoceros gracilis by flocculation using Chitosan

1st International Conference on Fisheries and Marine Science

Yamin, W.A.A.^1 ; Shaleh, S.R.M.^1 ; Ching, F.F.^1 ; Othman, R.^1 ; Manjaji-Matsumoto, M.^1 ; Mustafa, S.^1 ; Shigeharu, S.^2 ; Kandasamy, G.^3

Borneo Marine Research Institute, Universiti Malaysia Sabah, Sabah, Kota Kinabalu

88450, Malaysia^1

Fisheries Institute, Kinki University, 3153 Shirahama, Wakayama

649-2211, Japan^2

Department of Botany, Ayya Nadar Janaki Ammal College, Sivakasi, Tamil Nadu

626124, India^3

关键词: Aquaculture industry;    Chitosan concentration;    Culture conditions;    Ferric chloride;    Flocculating agents;    Flocculation efficiency;    Flocculation performance;    Growth conditions;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/236/1/012123/pdf
DOI  :  10.1088/1755-1315/236/1/012123

来源: IOP

【 摘 要 】

Flocculation is a method of harvesting microalgae by separating cells from the culture medium using flocculating agents. Ferric Chloride and alum are conventional flocculants that have the disadvantages of toxic residue. In this study, chitosan, a non-toxic flocculating agent is tested on Chaetoceros gracilis to determine the effect of culture conditions (salinity and cell density) towards the flocculation efficiency (FE). Marine diatom C. gracilis is cultured using Walne's Media for 8 days in a temperature controlled room and illuminated for 12 hours. Four different salinities (20, 25, 30, 35 ppt), four different cell densities (0.5, 2, 4, 6 x 106 cells mL-1), and six chitosan concentration (0, 15, 30, 45, 60, 75 ppm) are the parameters tested in this study. Measuring cylinder test is used to measure FE using spectrophotometer (OD750). From the results, salinity and cell density affect significantly the FE of the diatom. In 20 ppt culture, the FE was 89% after 50 minutes using 75 ppm of chitosan at a density of 4 x 106 cells mL-1. Growth conditions of the diatom culture have strong influences on the flocculation performances. The chitosan can be an option for non-toxic flocculants for concentrating live feeds in the aquaculture industry.