Isolation, characterization and synthesis of a novel pardaxin isoform

FEBS Letters

Adermann, Knut¹  Lazarovici, Philip⁴  Paul, Yasmin²  Bloch-Shilderman, Eugenia⁴  Hochman, Jacob³  Abu-Raya, Saleh⁴  Raida, Manfred¹  Wellhöner, Hans² 

[1] Niedersächsisches Institut für Peptid-Forschung (IPF), Feodor-Lynen-Str. 31, D-30625 Hannover, Germany;Institut für Toxikologie, Medizinische Hochschule Hannover, D-30625 Hannover, Germany;Department of Cell and Animal Biology, Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem 91904, Israel;Department of Pharmacology and Experimental Therapeutics, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem 91120, Israel

关键词: Cytolytic toxin;    Pardaxin;    Pardachirus marmoratus;    CID;    collision-induced dissociation;    ES-MS;    electrospray mass spectrometry;    Fmoc;    9-fluorenylmethoxycarbonyl;    HPLC;    high performance liquid chromatography;    LC/MS;    liquid chromatography/mass spectrometry;    RP;    reverse-phase;    TFA;    trifluoroacetic acid;   

DOI  :  10.1016/S0014-5793(98)01057-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the isolation of a novel pardaxin isoform from the toxic secretion of the Red Sea Moses sole (Pardachirus marmoratus). Mass spectrometrical analysis of the newly purified peptide revealed a different primary structure compared to the previously known pardaxin isoforms. Sequence analysis disclosed an aspartic acid residue instead of glycine at position 31 of the new isoform. According to the novel sequence, a synthetic Asp-31-peptide was compared with the native compound as well as with synthetic Gly-31-pardaxin. The isolated Asp-31-pardaxin isoform and its synthetic analog exhibited identical elution properties during reverse-phase HPLC, as well as similar dose-dependent lytic effects on human erythrocytes at a concentration of 10⁻⁶ to 10⁻⁵ M. The hemolytic activity of Asp-31-pardaxins was lower than that of Gly-31-pardaxin and no synergistic effect between these peptides was found. The additional negative charge introduced by Asp-31 is likely to affect the selectivity of pardaxin pores towards a variety of ions.

【 授权许可】

Unknown