Voltage‐dependent cationic channels formed by a cytolytic toxin produced by Gardnerella vaginalis

FEBS Letters

Virginio, Caterina¹  Zegarra-Moran, Olga²  Moran, Oscar²  Rottini, Giandomenico¹ 

[1] Istituto di Patologia Generale, Università di Trieste, Via A. Fleming 22, I-34127 Trieste, Italy;Laboratorio di Neurofisiologia, SISSA, Via Beirut 214, I-34014 Trieste, Italy

关键词: Cytolytic toxin;    Ionic channel;    Patch clamp;    Gardnerella vaginalis;   

DOI  :  10.1016/0014-5793(91)80617-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cytolytic toxin produced by G. vaginalis was incorporated in artificial membranes and giant liposomes. The toxin formed ionic channels when incorporated in lipid bilayers. The electrical properties of such channels were studied. Current records revealed a unitary conductance of 126 pS (in symmetrical 150 mM KCl). The open state probability of the cytolysin formed channels was a function of the applied membrane potential. The permeability ratio of cations to anions was estimated to be 6.5.

【 授权许可】

Unknown