Sequencing and synthesis of pardaxin, a polypeptide from the Red Sea Moses sole with ionophore activity

FEBS Letters

Lazarovici, Philip¹  Shai, Yechiel³  Shih, Yu-Liang⁴  Fox, Jay²  Edwards, Charles⁴  Caratsch, Carlo⁵ 

[1] Section on Growth Factors, NICHD, National Institutes of Health, Bethesda, MD 20892, USA;Dept of Microbiology, University of Virginia, School of Medicine, Box 441, Charlottesville, VA 22908, USA;Molecular, Cellular and Nutritional Endocrinology Branch, Bethesda, MD 20892, USA;Lab of Cell Biology and Genetics, NIDDK, National Institutes of Health, Bethesda, MD 20892, USA;Dept of Pharmacology, University of Zurich, Gloriastr. 32, CH-8006 Zurich, Switzerland

关键词: Pardaxin;    Presynaptic activity;    Neuromuscular junction;    Planar lipid bilayer;    Pore activity;    TFA;    trifluoroacetic acid;    HOBT;    hydroxybenzotriazole;    DCC;    dicyclohexylcarbodiimide;    PBS;    phosphatebuffered saline;    PX;    pardaxin;    HPLC;    high-performance liquid chromatography;    MEPP;    miniature end plate potential;    PE;    phosphatidylethanolamine;   

DOI  :  10.1016/0014-5793(88)81007-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Pardaxin, an amphipathic polypeptide secreted by the Red Sea flatfish Pardachirus marmoratus whose sequence is NH₂-G-F-F-A-L-I-P-K-I-I-S-S-P-L-F-K-T-L-L-S-A-V-G-S-A-L-S-S-S-G-G-Q-E, was synthesized by the solid-phase method. The structure was verified by sequencing. The synthetic polypeptide changed the resistance of lipid bilayers by forming pores. At 10⁻⁷−10⁻⁸ M, the synthetic pardaxin increased the frequency of the spontaneous release of quanta of acetylcholine at the neuromuscular junction by up to 100-fold, resembling the native product. Synthetic pardaxin seems to be a suitable tool for investigating the molecular structures underlying channel selectivity.

【 授权许可】

Unknown