Pardaxin induces aggregation but not fusion of phosphatidylserine vesicles

FEBS Letters

Lazarovici, Philip¹  Lelkes, Peter I.² 

[1] Section on Growth Factors, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA;Laboratory of Cell Biology and Genetics, National Institute of Diabetes, Digestive and Kidney Diseases and National Institute of National Institutes of Health, Bethesda, MD 20892, USA

关键词: Pardaxin;    Phosphatidylserine vesicle;    Phosphatidylserine aggregation;    N-NBD-PE;    N-4-nitrobenzo-2-oxa-1;    3-diazole phosphatidylethanolamine;    N-Rho-PE;    N-lissamine rhodamine-B-sulfonyl phosphatidylethanolamine;    ANTS;    8-aminonaphthalene 1;    3;    6-trisulfonate;    DPX;    p-xylene-bis-pyridinium bromide;    PC;    phosphatidylcholine;    PS;    phosphatidylserine;    PBS;    phosphate buffered saline;    PX;    pardaxin;    LUV;    large unilamellar liposome;    SUV;    small unilamellar liposome;   

DOI  :  10.1016/0014-5793(88)80656-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects on membranes of pardaxin, an amphipathic polypeptide, purified from the gland secretion of the Red Sea Moses sole flatfish Pardachirus marmoratus are dose-dependent and range from formation of voltage-gated, cation-selective pores to lysis. We have now investigated the interactions of pardaxin with small unilamellar liposomes. Light scattering showed that pardaxin (10⁻⁷–10⁻⁹M) mediated the aggregation of liposomes composed of phosphatidylserine but not of phosphatidylcholine. Aggregation of phosphatidylserine vesicles was impaired by vesicle depolarization. Furthermore, pardaxin-mediated aggregation between fluorescent-labeled PS vesicles was accompanied by leakage of the vesicle contents, and not by fusogenic process within the aggregates. We suggest that pardaxin is a unique polypeptide, that induces vesicle aggregation and membrane destabilization, but not membrane fusion; the mechanism of the aggregation activity of pardaxin is related to its amphipathic properties.

【 授权许可】

Unknown