In Vitro Environmental Stresses for Enhancing Withanolides Production in Physalis angulata L.

12th International Interdisciplinary Studies Seminar - Environmental Conservation and Education for Sustainable Development

生态环境科学

Mastuti, R.^1 ; Rosyidah, M.^2

Biology Department, Faculty of Mathematics and Natural Sciences, Brawijaya University, Malang, Indonesia^1

Biology Education Department, Faculty of Teacher Training and Education, Kadiri Islamic University, Kediri, Indonesia^2

关键词: Biotic elicitors;    Environmental stress;    Growth regulators;    HPLC;    Medicinal plants;    Secondary metabolites;    Shoot cultures;    withanolides;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/239/1/012011/pdf
DOI  :  10.1088/1755-1315/239/1/012011

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Production of secondary metabolites through in vitro culture can be increased by providing environmental stress conditions. Therefore, the objective of this study was to determine the effect of in vitro environment stress by the addition of elicitor on the withanolides production of shoots culture of P. angulata. Two weeks old shoot culture of P. angulata was elicited in either MS medium + biotic elicitor (25, 75, and 125 mg/L chitosan) or abiotic (PEG 2.5, 3, and 5%) for two weeks. The control was MS0 medium without the addition of growth regulators or elicitor. The results of HPLC analysis showed that chitosan elicitor had better effect than PEG. Chitosan was more effective in increasing withanolide content than PEG with a little inhibition of growth. Elicitation with PEG slightly increased withanolide with more inhibiting shoot growth. This result shows that stress manipulation in the in vitro environment through elicitation is potential strategy to improve withanolides production from shoot culture of P. angulata.