Location of phosphorylation site and DNA‐binding site of a positive regulator, OmpR, involved in activation of the osmoregulatory genes of Escherichia coli

FEBS Letters

Kato, Masashi²  Mizuno, Takeshi²  Nishimura, Yoshifumi¹  Aiba, Hirofumi²  Tate, Shin-ichi¹ 

[1] Faculty of Pharmaceutical Sciences, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan;Laboratory of Microbiology, School of Agriculture, Nagoya University, Chikusa-ku, Nagoya 464, Japan

关键词: Activator protein;    OmpR;    Phosphorylation;    DNA recognition;    (E. coli outer membrane);   

DOI  :  10.1016/0014-5793(89)80617-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The OmpR protein of Escherichia coli is a positive regulator involved in activation of the ompF and ompC genes which encode the major outer membrane proteins OmpF and OmpC, respectively. By employing recombinant DNA techniques, we isolated the N- and C-terminal halves of the OmpR molecule. From the results of biochemical analyses of these fragments, it was concluded that the N-terminal portion contains a site involved in phosphorylation by an OmpR-specific protein kinase EnvZ, whereas the C-terminal part possesses a DNA-binding site for the ompC and ompF promoters.

【 授权许可】

Unknown