Involvement of activator protein in the activation of tryptophan hydroxylase by cAMP‐dependent protein kinase

FEBS Letters

Fujisawa, Hitoshi¹  Okuno, Sachiko¹  Makita, Yoshio¹ 

[1] Department of Biochemistry, Asahikawa Medical College, Asahikawa 078, Japan

关键词: Tryptophan hydroxylase;    cAMP-dependent protein kinase;    Calmodulin-dependent protein kinase II;    Activator protein;    14-3-3 protein;    Rat brain;    PKA;    cAMP-dependent protein kinase;    CaM-kinase II;    calmodulin-dependent protein kinase II;    Mes;    4-morpholine-ethanesulfonic acid;    Hepes;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;    NSD-1015;    3-hydroxybenzylhydrazine;    6MPH4;    2-amino-4-hydroxy-6-methyltetrahydropteridine;    8Br-cAMP;    8-bromoadenosine 3';    5'-cyclic monophosphate;    db-cAMP;    N 6;    2'-O-dibutyryladenosine 3';    5'-cyclic monophosphate;   

DOI  :  10.1016/0014-5793(90)81004-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The tryptophan hydroxylase activity of the crude extract from rat brain stem was stimulated approximately 2-fold by incubation with cAMP analogues under protein phosphorylating conditions. The cAMP-dependent activation process of the enzyme needed not only cAMP-dependent protein kinase but also activator protein. The kinetic properties of the enzyme activated by cAMP-dependent protein kinase were very similar to those of the enzyme activated by calmodulin-dependent protein kinase II.

【 授权许可】

Unknown