New expression vectors for the fission yeast Schizosaccharomyces pombe

FEBS Letters

Bröker, Michael¹  Bäuml, Oskar¹ 

[1] Research Laboratories of Behringwerke AG, Postfach 1140, D-3550 Marburg, FRG

关键词: Expression vector;    Alcohol dehydrogenase;    Gene promoter;    Fission yeast;    Factor XIIIa;    (Schizosaccharomyces pombe;    Human);   

DOI  :  10.1016/0014-5793(89)80441-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A general expression vector (pMB332) for the fission yeast Schizosaccharomyces pombe was constructed. The heterologous gene expression is driven by the S. pombe alcohol dehydrogenase (adh) promoter. Transcription termination signals were isolated from the S. pombe actin gene. The vectors carry the Saccharomyces cerevisiae Ura3 gene, which complements the S. pombe ura4 mutation. The plasmid stability is conferred by the S. pombe ars and stb elements isolated from pFL120 [(1983) Cell 32, 371–377]. An ‘ATG’ vector (pMB340) was created, which allows the expression of protein fragments fused to a translational start codon downstream of the adh promoter. The function of this vector system is shown by the production of the human blood coagulation protein factor XIIIa.

【 授权许可】

Unknown