Analysis of dipeptidyl peptidase IV gene regulation in transgenic mice: DNA elements sufficient for promoter activity in the kidney, but not the intestine, reside on the proximal portion of the gene 5′‐flanking region

FEBS Letters

Erickson, Roger H.²  Gum, James R.¹  Rius, Jose L.²  Hicks, James W.²  Kim, Young S.¹ 

[1] Gastrointestinal Research Laboratory, Department of Veterans’ Affairs Medical Center, 4150 Clement Street, San Francisco, CA 94121, USA;Department of Medicine, University of California at San Francisco, San Francisco, CA, USA

关键词: Transgenic mouse;    Brush border peptidase;    Transcriptional regulation;    Gene promoter;    Promoter–reporter construct;    DPPIV;    dipeptidyl peptidase IV;    hGH;    human growth hormone;    HNF-1;    hepatic nuclear factor-1;    PCR;    polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(00)02013-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The dipeptidyl peptidase IV (DPPIV) gene encodes a brush border membrane exopeptidase that is expressed in a tissue-restricted fashion. To examine the regulation of DPPIV transcription in various tissues in vivo, we examined the expression of DPPIV 5′-flanking region (promoter)–human growth hormone reporter constructs in transgenic mice. These mice exhibited cell-type specific reporter expression in kidney. Surprisingly, however, only very low to non-detectable levels of reporter were found in small intestine. These results indicate that DNA elements sufficient for DPPIV expression in kidney, but not intestine, reside in the 5′-flanking region of the gene.

【 授权许可】

Unknown