Potency of papua local chickens as egg producers: a molecular review

1st International Conference of Animal Science and Technology 2018

Mu'In, Muh A.^1 ; Lumatauw, S.^1

Department of Animal Production, Faculty of Animal Science, University of Papua, Papua New Guinea^1

关键词: Agarose gel;    Blood samples;    DNA fragment;    DNA isolation;    Electrophoresis analysis;    Gene promoter;    Polymerase chain reaction methods;    Specific primers;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/247/1/012032/pdf
DOI  :  10.1088/1755-1315/247/1/012032

来源: IOP

【 摘 要 】

This research aims to evaluate potency of Papua Local Chickens as egg producers through allelic frequency estimation of 24-bp indel (insertion-deletion) on the prolactin gene promoter (cPRL) owned by the population. A total of 68 Papua Local Chickens (ALP) are used in this research, and blood samples are taken individually for DNA isolation. DNA fragment target size 130 or 154 bp contain the 24-bp indel locus is successfully amplified using Polymerase Chain Reaction (PCR) method with a pair of specific primer. Electrophoresis analysis on 1.5 % agarose gel indicates three genotypes: II, ID and DD with the frequency of 8.82 %, 41.18 % and 50.00 %, respectively. The I and D allele frequencies are 0.29 and 0.71. This result estimate shows that the I allele frequency is categorized medium in Papua Local Chickens population. Therefore, the Papua Local Chickens is potential in producing eggs. Their egg producer potency can be increased by eliminating the broodiness behaviour of the hens in the population or by increasing the I allele frequency through a controlled mating.