Identification of pork contamination in meatball using genetic marker mitochondrial DNA cytochrome b gene by duplex-PCR

International Conference On Food Science and Engineering 2016

Novianty, E.^1 ; Kartikasari, L.R.^1 ; Lee, J.H.^2 ; Cahyadi, M.^1

Department of Animal Science, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta

57126, Indonesia^1

Division of Animal and Dairy Science, Chungnam National University, Deajeon

34134, Korea, Republic of^2

关键词: Agarose gel;    Cytochrome b genes;    Duplex PCR;    Genetic markers;    High sensitivity;    Mitochondrial DNA;    Processed products;    Species-specific primers;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/193/1/012002/pdf
DOI  :  10.1088/1757-899X/193/1/012002

来源: IOP

【 摘 要 】

Meat based food products have a big opportunity to mix and adulterated with other meats. Muslim communities are prohibited to consume pork-containing product or other pig derivatives in food. Therefore, the high sensitivity, fast, cheap and accurate approach is needed to detect pig contamination in raw meat and meat-processed product such as meatball. The aim of this study was to identify pork contamination in meatball using genetic marker of mitochondrial DNA cytochrome b gene by duplex-PCR. Samples were prepared and designed by following the proportions 0, 1, 5, 10, 25% of pork in meatballs, respectively. The DNA genome was extracted from meatballs and polymerase chain reaction (PCR) was performed using species specific primer to isolate mt-DNA cytochrome b gene. The results showed that the DNA genome was successfully isolated from pork, beef, and contaminated meatballs. Furthermore, 2% agarose gels was able to visualize of duplex-PCR to identify pork contamination in meatballs up to very small proportion (1%). It can be concluded that duplex-PCR of mt-DNA cytochrome b gene was very sensitive to identify pork contamination in meatball with the presence of specific 398 bp DNA band.