Alcohol Dehydrogenase of Bacillus strain for Measuring Alcohol Electrochemically

3rd International Seminar On Sciences "Sciences On Precision And Sustainable Agriculture"

Iswantini, D.^1 ; Nurhidayat, N.^2 ; Ferit, H.^1

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor

16680, Indonesia^1

Division of Microbiology RandD for Biology, Indonesian Institute of Sciences, Jalan Raya Jakarta-Bogor Km 46, Cibinong Bogor

16911, Indonesia^2

关键词: Alcohol dehydrogenase;    Bacillus strain;    Bioreceptors;    Detection range;    Ethanol concentrations;    Measurement methods;    Oxidation currents;    Reduction values;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/58/1/012010/pdf
DOI  :  10.1088/1755-1315/58/1/012010

来源: IOP

【 摘 要 】

Alcohol dehydrogenase (ADH) was applied to produce alcohol biosensor. The enzyme was collected from cultured Bacillus sp. in solid media. From 6 tested isolates, bacteria from fermented rice grain (TST.A) showed the highest oxidation current which was further applied as the bioreceptor. Various ethanol concentrations was measured based on the increase of maximum oxidation current value. However, a reduction value was happened when the ethanol concentration was higher than 5%. Comparing the result of spectrophotometry measurement, R2value obtained from the biosensor measurement method was higher. The new proposed method resulted a wider detection range, from 0.1-5% of ethanol concentration. The result showed that biosensor method has big potency to be used as alcohol detector in foods or bevearages.