Evaluation of alcohol dehydrogenase and aldehyde dehydrogenase enzymes as bi-enzymatic anodes in a membraneless ethanol microfluidic fuel cell

15th International Conference on Micro and Nanotechnology for Power Generation and Energy Conversion Applications

物理学;能源学

Galindo-De-La-Rosa, J.^1 ; Arjona, N.^2 ; Arriaga, L.G.^3 ; Ledesma-García, J.^1 ; Guerra-Balcázar, M.^1

Facultad de Ingeniería, División de Investigación y Posgrado, Universidad Autónoma de Querétaro, Querétaro, Qro.

C.P. 76010, Mexico^1

Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Unidad Tijuana, Tijuana

C.P. 22444, Mexico^2

Centro de Investigación y Desarrollo Tecnológico en Electroquímica, Pedro-Escobedo-Qro.

C.P. 76703, Mexico^3

关键词: Alcohol dehydrogenase;    Aldehyde dehydrogenase;    Combined configuration;    Covalent binding;    Direct oxidation;    Ethanol electro-oxidation;    Microfluidic fuel cell;    Vertical positions;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/660/1/012131/pdf
DOI  :  10.1088/1742-6596/660/1/012131

来源: IOP

【 摘 要 】

Alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (AldH) enzymes were immobilized by covalent binding and used as the anode in a bi-enzymatic membraneless ethanol hybrid microfluidic fuel cell. The purpose of using both enzymes was to optimize the ethanol electro-oxidation reaction (EOR) by using ADH toward its direct oxidation and AldH for the oxidation of aldehydes as by-products of the EOR. For this reason, three enzymatic bioanode configurations were evaluated according with the location of enzymes: combined, vertical and horizontally separated. In the combined configuration, a current density of 16.3 mA cm-2, a voltage of 1.14 V and a power density of 7.02 mW cm-2were obtained. When enzymes were separately placed in a horizontal and vertical position the ocp drops to 0.94 V and to 0.68 V, respectively. The current density also falls to values of 13.63 and 5.05 mA cm-2. The decrease of cell performance of bioanodes with separated enzymes compared with the combined bioanode was of 31.7% and 86.87% for the horizontal and the vertical array.