FEBS Letters | |
Molecular cloning and chromosomal localization of a novel Drosophila protein phosphatase | |
Dombrádi, Viktor1  Axton, J.Myles2  Glover, David M.2  Cohen, Patricia T.W.1  | |
[1] MRC Protein Phosphorylation Group, Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, Scotland;CRC Eukaryotic Molecular Genetics Research Group, Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, England | |
关键词: Protein phosphatase; cDNA cloning; Nucleotide sequence; Amino acid sequence; (Drosophila melanogaster); PP; protein phosphatase; kb; kilobase; SSC; 0.15 M NaCl; 0.015 M sodium citrate; pH 7.0; | |
DOI : 10.1016/0014-5793(89)81377-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A 1.0 kilobase cDNA coding for the complete amino acid sequence of a putative protein phosphatase (314 amino acid residues, molecular mass 36 kDa) has been isolated from a Drosophila head cDNA library. The cDNA hybridises to a single site on the right arm of the second chromosome at cytological position 55A1–3. The deduced sequence of the protein, designated protein phosphatase-Y, is homologous to the catalytic subunits of Drosophila and rabbit protein phosphatase- 1α (64 and 59% identity, respectively) and rabbit protein phosphatase-2A (39% identity). These and other comparisons demonstrate that this novel enzyme is not the Drosophila counterpart of mammalian protein phosphatases 1, 2A, 2B, 2C or X.
【 授权许可】
Unknown
【 预 览 】
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RO201912020291928ZK.pdf | 420KB | download |