Molecular cloning and chromosomal localization of a novel Drosophila protein phosphatase

FEBS Letters

Dombrádi, Viktor¹  Axton, J.Myles²  Glover, David M.²  Cohen, Patricia T.W.¹ 

[1] MRC Protein Phosphorylation Group, Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, Scotland;CRC Eukaryotic Molecular Genetics Research Group, Department of Biochemistry, Imperial College of Science, Technology and Medicine, London SW7 2AZ, England

关键词: Protein phosphatase;    cDNA cloning;    Nucleotide sequence;    Amino acid sequence;    (Drosophila melanogaster);    PP;    protein phosphatase;    kb;    kilobase;    SSC;    0.15 M NaCl;    0.015 M sodium citrate;    pH 7.0;   

DOI  :  10.1016/0014-5793(89)81377-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 1.0 kilobase cDNA coding for the complete amino acid sequence of a putative protein phosphatase (314 amino acid residues, molecular mass 36 kDa) has been isolated from a Drosophila head cDNA library. The cDNA hybridises to a single site on the right arm of the second chromosome at cytological position 55A1–3. The deduced sequence of the protein, designated protein phosphatase-Y, is homologous to the catalytic subunits of Drosophila and rabbit protein phosphatase- 1α (64 and 59% identity, respectively) and rabbit protein phosphatase-2A (39% identity). These and other comparisons demonstrate that this novel enzyme is not the Drosophila counterpart of mammalian protein phosphatases 1, 2A, 2B, 2C or X.

【 授权许可】

Unknown