First Report of Tobacco Mosaic Virus on Cucumber [Cucumis sativus (L.)] in Java, Indonesia

2nd International Conference on Biosciences

Listihani^1 ; Hidayat, S.H.^1 ; Wiyono, S.^1 ; Damayanti, T.A.^1

Department of Plant Protection, Faculty of Agriculture, Bogor Agricultural University, Indonesia^1

关键词: Amino acid sequence;    CGMMV;    cucumber;    Cucumber green mottle mosaic virus;    Nucleotide sequence analysis;    TA-cloning vectors;    Tobacco mosaic virus;    Tobamovirus;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/197/1/012043/pdf
DOI  :  10.1088/1755-1315/197/1/012043

来源: IOP

【 摘 要 】

A mosaic and mottle symptom was observed on several cucumber cultivations in Java, Indonesia. The study was aimed to characterize the causal virus of typical Tobamovirus-like symptoms. Symptomatic leaves were collected randomly from each location in Nganjuk, Kediri, Tulungagung (East Java), Brebes and Klaten (Central Java), Kulon Progo (Yogyakarta), and Subang, Indramayu, Bogor (West Java). The diseases frequency was determined by dot blot immuno binding assays (DIBA) test using specific antiserum of Cucumber green mottle mosaic virus (CGMMV). Furthermore, the Tobamovirus was detected by RT-PCR, then cloned into TA-cloning vector, and the DNA was sequenced. The disease frequency of CGMMV in East Java, Central Java, Yogyakarta, and West Java was up to 24.44%, 9.09%, 0%, and 5.49%, respectively. CGMMV was unable to be amplified by specific primer, however it was able to be amplified by universal primer of Tobamovirus with sized ± 800 bp. Nucleotide sequence analysis of samples which gave positive reaction against CGMMV antisera were identified as Tobacco mosaic virus (TMV). The homology of nucleotide and amino acid sequences analysis ranged from 95.0-97.7% and 98.1-99.4%, respectively. This is the first report of TMV infection on cucumber in Indonesia.