Genetic diversity of the Cucumber green mottle mosaic virus and the development of RT-LAMP assay for its detection

Plant Protection Science

Beata Hasiów-Jaroszewska¹ 

关键词: CGMMV;    cucumber;    genetic variability;    identification;    LAMP method;   

DOI  :  10.17221/92/2018-PPS

学科分类：农业科学（综合）

来源: Czech Academy of Agricultural Sciences

【 摘 要 】

To analyse the genetic diversity of the Cucumber green mottle mosaic virus ( CGMMV) population in Poland and to establish the phylogenetic relationships between the Polish and other isolates described to date, 91 isolates were collected from cucumber plants. The analysis, based on coat protein (CP) gene, revealed the presence of two phylogenetic groups: one consisting of the Polish isolates collected in 2017 and those originated mainly from Asia region and the second including the Polish isolates collected in 2016 and the others from European countries. The sensitive, specific, and rapid one-step loop-mediated isothermal amplification assay was developed for the early detection of genetically diverse CGMMV isolates in seeds and plant material.

【 授权许可】

CC BY