Clone and Sequence Analysis of Cu/Zn-SOD Gene from Saccharum arundinaceum Retz.

2nd International Conference on Materials Science, Energy Technology and Environmental Engineering

材料科学;能源学;生态环境科学

Yao, Yanli^1 ; He, Junjun^1 ; Xing, Shulian^1 ; Hu, Xiaowen^1 ; Xu, Lei^1 ; Liu, Yang^1

Zhanjiang Experiment Station, Guangdong Dryland Water-saving Agric. Eng. and Technol. Res. Ctr./Trop. Dry Farming Research Center, Chinese Academy of Tropical Agricultural Sciences, Guangdong Zhanjiang

524013, China^1

关键词: Amino acid sequence;    Bioinformatics analysis;    Drought resistance;    Phylogenetic tree analysis;    Reactive oxygen species;    Stress-tolerance genes;    Superoxide dismutases;    Untranslated regions;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/81/1/012001/pdf
DOI  :  10.1088/1755-1315/81/1/012001

来源: IOP

【 摘 要 】

Saccharum arundinaceum has strong drought tolerance, the abundant stress tolerance gene resource could be used in sugarcane breeding programs. Superoxide dismutase (SOD) is a crucial enzyme in reactive oxygen species (ROS) action, which are likely to function as components of a stress-signaling pathway. To investigate the gene of SOD, by using a homologous cloning strategy, a Cu/Zn-SOD cDNA sequence(NCBI accession number XM- 002445626.1) in Sorghum was as probe to retrieve, aligned and spliced with Saccharum EST database, a full length of Cu/Zn-SOD gene named SaSOD-1b (NCBI accession number: KJ 002570) from Saccharum arundinaceum was cloned. The cloned gene sequence was 692bp, including 136bp 5 untranslated regions (UTR), 162bp ORF and 135bp 3 UTR, encoded 206 amino acids. By bioinformatics analysis, the cloned genic protein molecular weight was 20.78kDa, and pI was 5.32, the amino acid sequence owned the Cu/Zn-SOD conserved domain, and it had high similarity with other plants Cu/Zn-SOD. The phylogenetic tree analysis indicated that the cloned gene was most closely relative with Sorghum Cu/Zn-SOD gene, this study could laid a foundation for further understanding the relation between SOD and Saccharum arundinaceum drought resistance.