Viral subtyping of HIV-1 derived from infected, drug-naive individuals in Jakarta, Indonesia

3rd Annual Applied Science and Engineering Conference

工业技术;自然科学

Witaningrum, A.M.^1 ; Khairunisa, S.Q.^1 ; Ueda, S.^1,2,3 ; Yunifiar, M.Q.^1 ; Indriati, D.W.^1,5 ; Kotaki, T.^2 ; Rusli, A.^4 ; Nasronudin, N.^1,6 ; Kameoka, M.^2,3

Indonesia-Japan Collaborative Research Centre for Emerging and Re-emerging Infectious Diseases, Institute of Tropical Disease, Airlangga University, Surabaya, Indonesia^1

Departemen of Public Health, Kobe University, Graduate School of Health Sciences, Hyogo, Japan^2

Centre for Infectious Diseases, Kobe University, Graduate School of Medicine, Hyogo, Japan^3

Sulianti Saroso Hospital, Jakarta, Indonesia^4

Department of Health, Vocational Faculty, Airlangga University, Surabaya, Indonesia^5

Airlangga Hospital, Airlangga University, Surabaya, Indonesia^6

关键词: Acquired immune deficiency syndrome;    Epidemiological studies;    Gene fragments;    HIV-1 infections;    Human immunodeficiency virus;    Peripheral blood;    Phylogenetic tree analysis;    Subtypings;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/434/1/012321/pdf
DOI  :  10.1088/1757-899X/434/1/012321

来源: IOP

【 摘 要 】

Human immunodeficiency virus (HIV) infection and acquired immune deficiency syndrome (AIDS) (HIV/AIDS) remain a serious health problem in Indonesia. Jakarta has the largest number of individuals infected with HIV type 1 (HIV-1) in Indonesia; however, data on viral subtyping of HIV-1 is limited. We conducted a molecular epidemiological study on HIV-1 genes derived from infected, drug-naive individuals residing in Jakarta, the capital of Indonesia with large communities of HIV-infected individuals. To determine the distribution of HIV-1 subtypes circulating in Jakarta, 43 HIV-1-infected individuals were recruited in Jakarta, Indonesia. HIV-1 gag and envgenes were amplified from peripheral blood samples derived from study participants. Viral subtyping using the Recombinant Identification Program (RIP) and phylogenetic tree analysis were conducted. RIP and phylogenetic tree analysis revealed that CRF01-AE was the dominant subtype in Jakarta (40%), followed by recombinant viruses containing CRF01-AE and subtype B gene fragments (7%) and subtype B (7%). This study reported the emergence of CRF 01-AE as predominant subtype among HIV-1-infected, drug-naïve individuals in Jakarta, Indonesia. Therefore, continuous surveillance is required in order to detect the emergence of HIV-1-infection.