Gardenia jasminoides Encodes an Inhibitor-2 Protein for Protein Phosphatase Type 1

2nd International Conference on Materials Science, Energy Technology and Environmental Engineering

材料科学;能源学;生态环境科学

Gao, Lan^1 ; Li, Hao-Ming^1

School of Basic Courses, Guangdong Pharmaceutical University, Guangzhou Higher Education Mega Center, Guangzhou

510006, China^1

关键词: Bioinformatics analysis;    Carbohydrate metabolism;    Cell-cycle progression;    Muscle contractions;    Phosphorylation sites;    Protein phosphatase;    Protein phosphatase-1;    Three-dimensional model;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/81/1/012040/pdf
DOI  :  10.1088/1755-1315/81/1/012040

来源: IOP

【 摘 要 】

Protein phosphatase-1 (PP1) regulates diverse, essential cellular processes such as cell cycle progression, protein synthesis, muscle contraction, carbohydrate metabolism, transcription and neuronal signaling. Inhibitor-2 (I-2) can inhibit the activity of PP1 and has been found in diverse organisms. In this work, a Gardenia jasminoides fruit cDNA library was constructed, and the GjI-2 cDNA was isolated from the cDNA library by sequencing method. The GjI-2 cDNA contains a predicted 543 bp open reading frame that encodes 180 amino acids. The bioinformatics analysis suggested that the GjI-2 has conserved PP1c binding motif, and contains a conserved phosphorylation site, which is important in regulation of its activity. The three-dimensional model structure of GjI-2 was buite, its similar with the structure of I-2 from mouse. The results suggest that GjI-2 has relatively conserved RVxF, FxxR/KxR/K and HYNE motif, and these motifs are involved in interaction with PP1.