Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of a type‐2A protein phosphatase

FEBS Letters

da Cruz e Silva, Odete B.¹  Cohen, Patricia T.W.¹  Campbell, David G.¹  Alemany, Susana¹ 

[1] Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, Scotland

关键词: Protein phosphatase;    cDNA cloning;    Nucleotide sequence;    Amino acid sequence;    Sequence homology;   

DOI  :  10.1016/0014-5793(87)80966-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 2.5 kb clone containing the full-length coding sequence of a type-2A protein phosphatase catalytic subunit has been isolated from a rabbit skeletal muscle cDNA library constructed in λgt10. The sequence of the protein deduced from the cDNA contains 309 residues (35.58 kDa). A major mRNA species at 2.0 kb and a minor component at 2.8 kb were visualized by Northern blotting in both skeletal muscle and liver. The type-2A enzyme showed weak homology with mammalian alkaline phosphatases between residues 55 and 95. The protein sequence of the type-2A phosphatase from rabbit skeletal muscle differs from that reported for the bovine adrenal enzyme in three regions.

【 授权许可】

Unknown