Electro‐stimulated transformation of E.coli cells pretreated by EDTA solution

FEBS Letters

Cymbalyuk, E.S.¹  Chizmadzhev, Yu.A.¹  Chernomordik, L.V.¹  Broude, N.E.² 

[1] A.N. Frumkin Institute of Electrochemistry, USSR Academy of Sciences, Leninsky prospect, 31, 117071 Moscow USSR;M.M. Shemyakin Institute of Bioorganic Chemistry, USSR Academy of Sciences, Moscow, USSR

关键词: Electrotransfection;    Electroporation;    (E.coli);   

DOI  :  10.1016/0014-5793(88)81334-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The phenomenon of transformation of E.coli cells under electric treatment has been studied. The cells of strains MH 1, HB 101 and DH 1 after EDTA treatment in an isotonic medium were transformed with DNA pBR322 by applying a single exponential pulse (E = 10 kV/cm, T = 1.5 ms) to the suspension. The maximum transformation efficiency obtained was 4 × 10⁶ colonies/μg DNA. The maximum transformation frequency was 0.4% at a DNA concentration of 15 μg/ml.

【 授权许可】

Unknown