Design, modelling and preliminary characterisation of microneedle-based electrodes for tissue electroporation in vivo

27th Micromechanics and Microsystems Europe Workshop

物理学;力学

O'Mahony, Conor^1 ; Houlihan, Ruth^1 ; Grygoryev, Konstantin^1 ; Ning, Zhenfei^2 ; Williams, John^2 ; Moore, Tom^2

Tyndall National Institute, University College Cork, Cork, Ireland^1

School of Biochemistry and Cell Biology, University College, Cork, Ireland^2

关键词: DNA vectors;    Electroporation;    GFP expression;    Green fluorescent protein;    In-vivo;    Microneedles;    Transgenic mice;    Voltage supply;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/757/1/012040/pdf
DOI  :  10.1088/1742-6596/757/1/012040

学科分类：力学，机械学

来源: IOP

【 摘 要 】

We analysed the use of microneedle-based electrodes to enhance electroporation of mouse testis with DNA vectors for production of transgenic mice. Different microneedle formats were developed and tested, and we ultimately used electrodes based on arrays of 500 μm tall microneedles. In a series of experiments involving injection of a DNA vector expressing Green Fluorescent Protein (GFP) and electroporation using microneedle electrodes and a commercially available voltage supply, we compared the performance of flat and microneedle electrodes by measuring GFP expression at various timepoints after electroporation. Our main finding, supported by both experimental and simulated data, is that needles significantly enhanced electroporation of testis.