Electroporation‐mediated gene transfer in cardiac tissue

FEBS Letters

Byrne, Barry J¹  Tung, Leslie²  Harrison, Rachel L² 

[1] Department of Pediatrics and Molecular Genetics and Microbiology, Gene Therapy Center, University of Florida, PO Box 100296, Gainesville, FL 32610, USA;Department of Biomedical Engineering, The Johns Hopkins University School of Medicine, 720 Rutland Avenue, Baltimore, MD 21205, USA

关键词: Electroporation;    Gene transfer;    Green fluorescent protein;    Luciferase;    Embryonic chick heart;   

DOI  :  10.1016/S0014-5793(98)00987-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Delivery of genes or macromolecules to cardiovascular tissues provides new therapeutic opportunities for the treatment of many acquired and inherited diseases. To investigate electroporation as a delivery method in cardiac tissue, embryonic chick hearts were studied for uptake of propidium iodide (PI) or DNA encoding either green fluorescent protein (GFP) or luciferase following electrical shock. PI uptake increased monotonically from 6% of heart tissue after 3 shocks to 77% with 12 shocks. GFP and luciferase expression varied in proportion to shock number, with detectable levels in all electrically treated hearts. Thus, electroporation promotes uptake of PI and DNA in cardiac tissue, suggesting further application of this method for therapeutic genes.

【 授权许可】

Unknown