In vivo gene electroinjection and expression in rat liver

FEBS Letters

Nicolau, Claude¹  Jaroszeski, Mark⁴  Moradpour, Darius³  Wands, Jack³  Atkin, Andrew¹  Gilbert, Richard²  Heller, Richard⁴ 

[1] Center for Blood Research Laboratories, Harvard Medical School, 1256 Soldiers Field Road, Boston, MA 02135, USA;Department of Chemical Engineering, College of Engineering, University of South Florida, Tampa, FL 33612, USA;Molecular Hepatology Laboratory, Massachusetts General Hospital Cancer Center, Harvard Medical School, Charlestown, MA 02129, USA;Department of Surgery, College of Medicine, University of South Florida, Tampa, FL 33612, USA

关键词: Electroporation;    Gene transfer;    Rat liver;    Luciferase;    Efficiency;    expression;    β-gal;    β-galactosidase;    Luc;    firefly lucirefase;    C12 FDG;    5-dodecanoylamino-fluorescein di-β-galactopyranoside;   

DOI  :  10.1016/0014-5793(96)00590-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In vivo targeted gene transfer by non-viral vectors is subjected to anatomical constraints depending on the route of administration. Transfection efficiency and gene expression in vivo using non-viral vectors is also relatively low. We report that in vivo electropermeabilization of the liver tissue of rats in the presence of genes encoding luciferase or β-galactosidase resulted in the strong expression of these genetic markers in rat liver cells. About 30–40% of the rat liver cells electroporated expressed the β-galactosidase genetic marker 48 h after electroporation. The marker expression was also detected at least 21 days after transfection at about 5% of the level 48 h after electroporation. The results indicate that gene transfer by electroporation in vivo may avoid anatomical constraints and low transfection efficiency.

【 授权许可】

Unknown