The effects of different concentrations of ccBA-GFP promoter with electroporation methods on the quality of koi sperm (Cyprinus carpio var. koi)

Asean-Fen International Fisheries Symposium - 2017

Soeprijanto, A.^1 ; Aisyah, D.^2

Faculty of Fisheries and Marine Science, Brawijaya University, Malang

65145, Indonesia^1

Master Programme. Faculty of Fisheries and Marine Science, Brawijaya University, Malang

65145, Indonesia^2

关键词: Cyprinus carpio;    Electroporation;    Fertilization rates;    Life-sciences;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/137/1/012025/pdf
DOI  :  10.1088/1755-1315/137/1/012025

来源: IOP

【 摘 要 】

The effectiveness of the use of promoter concentration which will be inserted into the Koi sperm as the medium of gene transfer is important. The objective of this research is to find out the influence of the adding of different concentrations of the ccBA-GFP promoter with electroporation methods to the motility, viability and the fertilization rate of the Koi sperm. This study was conducted at Central Lab of Life Sciences Brawijaya University in April 2017. Electroporation methods were conducted by using 30-volt voltage, 4 times shocks with 0.5 seconds per shock. The treatment of different concentration was done through 3 types of ccBA-GFP promoter concentration, namely: 10 ng/μl, 30 ng/μl, and 50 ng/μl. The best motility percentage with the score of 4 is at the treatment A (10ng/μl concentration), the best viability percentage is 77.83 % at the treatment A (10 ng/μl concentration) and the best fertilization rate is 73.09 % at the treatment A (10 ng/μl concentration). The result shows that there is a relationship between the treatment given to the motility and viability of the Koi sperm, at which, the higher the shocks, the lower the percentage of the motility and viability of the Koi sperm.