Direct monitoring of photon induced isomerization, dissociation and electron detachment of the green fluorescent protein chromophore anion

30th International Conference on Photonic, Electronic, and Atomic Collisions

Carrascosa, Eduardo^1 ; Scholz, Michael S.^1 ; Bull, James N.^1 ; Bieske, Evan J.^1

School of Chemistry, University of Melbourne, Parkville

VIC

3010, Australia^1

关键词: Biomolecular ions;    Direct monitoring;    Electron detachments;    Experimental demonstrations;    Green fluorescent protein;    Multi-photon absorption;    Photo-detachment;    Single-photon absorptions;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/875/4/032005/pdf
DOI  :  10.1088/1742-6596/875/4/032005

来源: IOP

【 摘 要 】

We present the first experimental demonstration of Z ↔ E photoisomerization the GFP chromophore anion, HBDI-, in the gas phase. In the single photon absorption regime, the photoisomerization action spectra show two maxima at 480 nm and 455 nm. In the multiphoton absorption regime, photodissociation and photodetachment channels modify the appearance of the photoisomerization band. This work provides a new approach to characterize photoisomerization pathways in biomolecular ions.