Palindromic units from E. coli as binding sites for a chromoid‐associated protein

FEBS Letters

Dassa, Elie¹  Perrin, David¹  Gilson, Eric¹  Szmelcman, Sevec¹  Hofnung, Maurice¹  Clement, Jean-Marie¹ 

[1] Unité de Programmation Moléculaire et Toxicologie Génétique, CNRS UA271 INSERM U163, Institut Pasteur, 28 rue du Docteur Roux, Paris 75015, France

关键词: (E.coli);    repetitive DNA sequence;    DNA-binding protein;    Chromoid structure;   

DOI  :  10.1016/0014-5793(86)81005-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Several hundred copies of a highly conserved extragenic palindromic sequence, 20–40 nucleotides long, exist along the chromosome of E. coli and S. typhimurium. These have been defined as palindromic units (PU) or repetitive extragenic palindromes (REP). No general function for PUs has been identified. In the present work, we provide data showing that a protein associated with a chromoid extract of E. coli protects PU DNA against exonuclease III digestion. This provides the first experimental evidence that PU constitutes binding sites for a chromoid-associated protein. This result supports the hypothesis that PUs could play a role in the structure of the bacterial chromoid.

【 授权许可】

Unknown