Is translation inhibited by noncognate ternary complexes?

FEBS Letters

Ehrenberg, Måns¹  Bilgin, Neşe¹  Kurland, Charles¹ 

[1] Dept of Molecular Biology, Uppsala University, The Biomedical Center, Box 590, S-751 24 Uppsala, Sweden

关键词: Protein synthesis;    Translational regulation;    Ribosomal A-site;    (E.coli);   

DOI  :  10.1016/0014-5793(88)81362-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We studied the influence of an error-prone isoacceptor (tRNA^Leu ₄), as well as an intermediate (tRNA^Leu ₂) and a weak (tRNA^Val) competitor of tRNA^Phe on the poly(Phe) synthesis rate. Even at very high excess concentrations of these noncognate ternary complexes there was no significant effect on the translation rate. Our result argues against the assertion that in vivo translation is slowed down by noncognate tRNA and favours the hypothesis that the incorrect ternary complex concentrations are too low to saturate the ribosomes in vivo.

【 授权许可】

Unknown