O2 −dependent lipid peroxidation does not affect the molecular order in hepatoma microsomes

FEBS Letters

Galeotti, T.¹  Masotti, L.⁴  Borrello, S.¹  Minotti, G.¹  Casali, E.⁴  Arcioni, A.³  Zannoni, C.³  Cavatorta, P.²  Ferrari, M.B.⁴ 

[1] Institute of General Pathology, Catholic University, Largo F. Vito 1, 00168 Roma RM, Italy;Department of Physics - GNCB, University of Parma, Via D'Azeglio 85, 43100 Parma PR, Italy;Institute of Physical Chemistry, University of Bologna, Viale Risorgimento 4, 40136 Bologna BO, Italy;Institute of Biological Chemistry, University of Parma, Viale Gramsci 14, 43100 Parma PR, Italy

关键词: (Morris hepatoma 3924A);    Microsome;    Lipid peroxidation;    Lipid order;    Fluorescence;   

DOI  :  10.1016/0014-5793(86)80425-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Microsomal membranes from rat liver and from the fast-growing Morris hepatoma 3942A have been peroxidized to different extents and the order parameter of the membranes measured by fluorescence depolarization of the probe 1,6-diphenyl-1,3,5-hexatriene. The data have been analysed by applying a mathematical approach that takes into account simultaneously static and dynamic fluorescence parameters. It appears that tumour membranes are more ordered than the control and their order parameter does not increase with greater exposure to the action of O⁻ ₂ radicals in contrast to liver membranes. The fatty acid composition of the membrane lipids has been studied under different experimental conditions and correlated to the behaviour of the physical parameter.

【 授权许可】

Unknown