Melatonin prevents changes in microsomal membrane fluidity during induced lipid peroxidation

FEBS Letters

Escames, Germaine¹  Yu, Byung P²  Reiter, Russel J¹  Guerrero, Juan M¹  Muñoz-Hoyos, Antonio¹  Garcı́a, Joaquı́n J¹  Oh, Chang S¹ 

[1] Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78284-7762, USA;Department of Physiology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78284-7762, USA

关键词: Melatonin;    Antioxidant;    Free radical;    Membrane fluidity;    Lipid peroxidation;    Microsome;    MDA;    malonaldehyde;    4-HDA;    4-hydroxyalkenals;    LOO·;    peroxyl radical;    ·OH;    hydroxyl radical;    TMA-DPH;    1-(4-trimethylammoniumphenyl)-6-phenyl-1;    3;    5-hexatriene-p-toluene-sulfonate;    THF;    tetrahydrofuran;   

DOI  :  10.1016/S0014-5793(97)00447-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We tested the effect of melatonin on membrane fluidity in microsomes of a rat liver model in which lipid peroxidation was induced by the addition of FeCl₃, ADP and NADPH. Membrane fluidity was monitored using fluorescence spectroscopy and lipid peroxidation was estimated by quantifying malonaldehyde (MDA)+4-hydroxyalkenals (4-HDA) concentrations following the induction of lipid peroxidation with and without pre-incubation with melatonin (1 μM–3 mM). Membrane rigidity increased during induced lipid peroxidation while melatonin reduced in a concentration-dependent manner both membrane rigidity and MDA+4-HDA generation. Melatonin's protective effect may relate to its known ability to scavenge free radicals and function as an antioxidant.

【 授权许可】

Unknown