Inhibition of adriamycin‐promoted microsomal lipid peroxidation by β‐carotene, α‐tocopherol and retinol at high and low oxygen partial pressures

FEBS Letters

Winterbourn, Christine C.¹  Vile, Glenn F.¹ 

[1] Department of Pathology, Christchurch School of Medicine, Christchurch Hospital, Christchurch, New Zealand

关键词: Lipid peroxidation;    Microsome;    Adriamycin;    β-Carotene;    α-Tocopherol;   

DOI  :  10.1016/0014-5793(88)80511-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Iron-dependent peroxidation of rat liver microsomes, enhanced by adriamycin, was measured in the presence of increasing concentrations of α-tocopherol, β-carotene and retinol at low and high pO₂. β-Carotene and α-tocopherol inhibited lipid peroxidation by more than 60% when present at concentrations greater than 50 nmol/mg microsomal protein at both high and low pO₂. Retinol inhibited peroxidation by 39% at concentrations greater than 100 nmol/mg microsomal protein. This maximal level of inhibition by retinol was unaltered by pO₂. However, β-carotene was more effective than α-tocopherol or retinol at a pO₂ of 4 mmHg, whereas α-tocopherol was more effective under aerobic conditions. Since adriamycin-dependent lipid peroxidation is maximal at low pO₂, β-carotene may play a role in protecting against this process.

【 授权许可】

Unknown