Stimulation of parathyroid hormone secretion by phorbol esters is associated with a decrease of cytosolic calcium

FEBS Letters

Muff, R.¹  Fischer, J.A.¹ 

[1] Research Laboratory for Calcium Metabolism, Departments of Orthopedic Surgery and Medicine, University of Zurich, Forchstrasse 340, 8008 Zurich, Switzerland

关键词: Parathyroid hormone;    Phorbol ester;    Cytosolic calcium;    DMSO;    dimethyl sulfoxide;    FDD;    phorbol-12;    13-didecanoate;    PTH;    parathyroid hormone;    TPA;    12-O-tetradecanoylphorbol-13-acetate;   

DOI  :  10.1016/0014-5793(86)80087-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Unlike in other endocrine systems calcium inhibits parathyroid hormone (PTH) secretion and this inhibition is paralleled by a rise of cytosolic calcium concentration ([Ca]_i). Because of evidence that diglyceride levels and protein kinase C activity are also decreased by high extracellular calcium we have investigated the effects of 12-O-tetradecanoylphorbol-13-acetate (TPA), an activator of protein kinase C, on [Ca]_i and PTH secretion using dispersed bovine parathyroid cells. At 1.5 mM medium calcium TPA enhanced PTH secretion and caused reduction of [Ca]_i from 639 ± 36 nM (SE) to 335 ± 21 nM (P < 0.001); at 0.5 mM calcium TPA was ineffective. Moreover, TPA suppressed the rise of [Ca]_i evoked by high extracellular calcium. Thus TPA presumably stimulates PTH secretion via activation of protein kinase C, and the lowering of [Ca]_i may be a secondary event related to diglyceride availability.

【 授权许可】

Unknown