Immunological evidence that insulin activates protein kinase C in BC3H‐1 myocytes

FEBS Letters

Farese, Robert V.¹  Acevedo-Duncan, Mildred¹  Cooper, Denise R.¹  Standaert, Mary L.¹ 

[1] James A. Haley Veterans' Hospital and Departments of Internal Medicine and Biochemistry, University, of South Florida, College of Medicine, Tampa, FL 33612, USA

关键词: Insuline Protein kinase C;    Myocyte: Diacylglycerol;    Phospholipid;    Phorbol ester;    PKC;    protein kinase C;    DAG;    diacylglycerol;    TPA;    12-O-tetradecanoyl phorbol-13-acetate;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    PMSF;    phenylmethylsulfonyl fluoride;    DMSO;    dimethyl sulfoxide;   

DOI  :  10.1016/0014-5793(89)81186-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Effects of insulin on immunoreactive protein kinase C were examined in BC3H-1 myocytes. Insulin provoked rapid dose-dependent decreases in cytosolic enzyme, and transient increases and subsequent decreases in membrane-associated enzyme. Phorbol esters provoked similar changes. Our findings suggest that insulin provokes the translocative activation of protein kinase C.

【 授权许可】

Unknown