Sperm capacitation is, after all, a prerequisite for both partial and complete acrosome reaction

FEBS Letters

Jaiswal, Bijay S¹  Tur-Kaspa, Ilan¹  Cohen-Dayag, Anat¹  Eisenbach, Michael¹ 

[1]Department of Biological Chemistry, The Weizmann Institute of Science, 76100 Rehovot, Israel

关键词: Capacitation;    Acrosome reaction;    Albumin;    Human sperm;    A23187;    Phorbol ester;    AR;    acrosome reaction;    BWW;    Biggers;    Whitten and Whittingham medium;    DMSO;    dimethyl sulfoxide;    FITC;    fluorescein isothiocyanate;    NCM;    non-capacitating medium;    PMA;    phorbol myristate ester;    PSA;    Pisum sativum agglutinin;   

DOI  :  10.1016/S0014-5793(98)00455-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The acrosome reaction (AR) – an essential step in mammalian fertilization – can occur, according to the consensus, only in capacitated spermatozoa. In apparent contrast, recent reports have demonstrated that human spermatozoa incubated in vitro in an albumin-free medium and therefore believed to be non-capacitated, do undergo the AR. With the aim of determining unequivocally whether or not capacitation is required for the AR and whether albumin is essential for capacitation, we compared the potential to undergo partial and complete AR (induced by phorbol myristate ester or by the Ca²⁺ ionophore A23187) between human spermatozoa incubated in a capacitating medium, albumin-free medium, and non-capacitating medium. The results clearly demonstrate that capacitation is, after all, a prerequisite for both partial and complete AR. Albumin, on the other hand, is essential only for acquiring the capacity to undergo complete, not partial AR.

【 授权许可】

Unknown