Bicarbonate/CO2 induces rapid activation of phospholipase A2 and renders boar spermatozoa capable of undergoing acrosomal exocytosis in response to progesterone

FEBS Letters

Roldan, E.R.S.¹  Vazquez, J.M.¹ 

[1] Department of Development and Signalling, The Babraham Institute, Cambridge CB2 4AT, UK

关键词: Phospholipase A2;    Capacitation;    Acrosome reaction;    Exocytosis;    Progesterone;    PLA2;    phospholipase A2;    PC;    phosphatidylcholine;    PS;    phosphatidylserine;   

DOI  :  10.1016/0014-5793(96)01110-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We tested whether capacitation, a phenomenon that renders spermatozoa capable of undergoing acrosomal exocytosis, may be completed rapidly after a short exposure to bicarbonate/CO₂. We found that, in the presence of Ca²⁺, a 10-min exposure of boar spermatozoa to bicarbonate led to a partial activation of phospholipase A₂, primed spermatozoa for a major subsequent activation of this enzyme upon stimulation with progesterone and furthermore rendered spermatozoa capable of undergoing exocytosis in response to this steroid. These results suggest that capacitation may be completed in a relatively short period of time and open up new possibilities for unravelling molecular mechanisms underlying this process.

【 授权许可】

Unknown