Phorbol ester stimulates amylase secretion from rat parotid cells

FEBS Letters

Ichida, Tokuro¹  Takuma, Taishin¹ 

[1] Department of Oral Biochemistry, School of Dentistry, Higashi Nippon Gakuen University, Tobetsu, Hokkaido 061-02, Japan

关键词: Phorbol ester;    Protein kinase C;    cyclic AMP;    Ca2+ ionophore;    Amylase secretion;    (Parotid gland);    PMA;    phorbol 12-myristate 13-acetate;    db-cAMP;    N 6;    O 2'-dibutyryl adenosine 3';    5'-monophosphate;    DMSO;    dimethyl sulfoxide;   

DOI  :  10.1016/0014-5793(86)81222-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phorbol myristate acetate (PMA), a potent activator of Ca²⁺- and phospholipid-dependent protein kinase (protein kinase C), evoked amylase release from rat parotid cells. In dose-response studies, PMA stimulated amylase release independently of db-cAMP, but potentiated the effect of carbachol. PMA and A23187, a Ca²⁺ ionophore, synergistically increased amylase release. The maximum effect of carbachol was further enhanced by PMA but not by A23187, suggesting that protein kinase C is not fully activated by the muscarinic-cholinergic agonist under the condition where calcium is fully utilized for amylase secretion.

【 授权许可】

Unknown