Antibody‐induced cAMP accumulation in splenocytes from athymic nude mice

FEBS Letters

Scarpa, Antonio¹  Wiener, E.C.¹  Griffor, M.C.¹ 

[1] Case Western Reserve University, Department of Physiologyy and Biophysics, Cleveland, OH 44106, USA

关键词: Lymphocyte;    Antibody;    cyclic AMP;    Protein kinase C;    Phorbol ester;    Forskolin;    cAMP;    adenosine-3′;    5′-cyclic phosphoric acid;    anti-Ig;    goat anti-mouse IgM μ-chain specific;    TPA;    12-O-tetradecanoylphorbol-13-acetate;    IP3;    inositol 1;    4;    5-trisphosphate;    PIP2;    phosphatidylinositol 4;    5-bisphosphate;    125 I-ScAMP;    2′-O-succinyl ([125-I]iodotyrosine methyl ester)-adenosine-3′;    5′-cyclic phosphoric acid;    BSA;    bovine serum albumin;    IBMX;    isobutyl-3-methyl-1-xanthine;    PEG-8000;    polyethylene glycol-8000;    PGE1;    prostaglandin E1;    diC8;    sn-1;    2-dioctanoylglycerol;    4α-PDD;    4-α-phorbol-12;    13-didecanoate;   

DOI  :  10.1016/0014-5793(87)80417-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Products from the hydrolysis of phosphatidylinositol 4,5-bisphosphate (IP₃) can increase and/or potentiate cAMP accumulation in a variety of cells. Antibody to surface immunoglobulins activates IP₃ hydrolysis in B-lymphocytes. In this study we have examined whether anti-Ig also stimulated and/or potentiated increases in the cAMP levels of splenocytes from athymic nude mice. Furthermore, since TPA potentiates anti-Ig-induced DNA synthesis and cAMP modulates DNA synthesis, the effects of TPA on any anti-Ig-induced changes in cAMP were also studied. Antibody (25 μg/ml) stimulated a rapid rise in cAMP which increased from 250 fmol/10⁶ cells to 400 fmol/10⁶ cells within 1 min and then subsided to 310 fmol/10⁶ cells by 10 min. TPA (96 nM) suppressed the anti-Ig-induced cAMP accumulation at 1 min by 60%, but potentiated the forskolin (114 μM)-induced rise by 151%. Two other activators of protein kinase C, dioctanoylglycerol (5 μM), and anti-Ig (25 μg/ml), also potentiated the forskolin response by 198% and 52%, respectively. These results suggest that modulation of the adenylate cyclase system by anti-Ig may act in concert with cytokines and/or prostaglandins secreted by other lymphoid cells to define the state of proliferation or differentiation in B-cells.

【 授权许可】

Unknown