12‐O‐Tetradecanoyl phorbol‐13‐acetate interferes with germination of Phycomyces blakesleeanus sporangiospores

FEBS Letters

Martinez-Cadena, Guadalupe¹  Garcia-Soto, Jesus¹  Carrillo-Rayas, Teresa¹ 

[1] Instituto de Investigacion en Biologia Experimental, Facultad de Quimica, Universidad de Guanajuato, Apartado Postal 187, Guanajuato, Gto. 36000, Mexico

关键词: Spore germination;    Protein kinase C;    Phorbol ester;    cyclic AMP;    (Phycomyces blakesleeanus);    PKC;    protein kinase C;    DG;    diacylglycerol;    TPA;    12-O-tetradecanoyl phorbol-13-acetate;    4αPDD;    4α-phorbol-12;    13-didecanoate;    OAG;    1-oleoyl-2-acetylglycerol;    dbcAMP;    dibutyryl cyclic AMP;    PS;    phosphatidylserine;   

DOI  :  10.1016/0014-5793(88)80528-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The presence of protein kinase C (PKC), a key enzyme in signal transduction, has not been investigated in fungal cells. The phorbol ester TPA, an activator of PKC, may be used as an indicator of the presence and role of PKC in Phycomyces blakesleeanus spores. Activation of spore germination by acetate was prevented by 6 nM TPA. The TPA analog 4αPDD, an ineffective activator of PKC, did not affect spore germination. 3 mM dbcAMP, on the other hand, reversed the inhibition of germination caused by TPA. TPA-stimulated protein kinase activity was detected in spores. The possible relationship between PKC and the increased levels of cAMP that accompany the induction of spore germination is discussed.

【 授权许可】

Unknown