Effect of DMSO concentration on the quality of goat fetus fibroblast cell cultured in vitro

9th Annual Basic Science International Conference 2019

自然科学（总论）

Karima, H.N.^1 ; Susilawati, Trinil^2 ; Djati, M.S.^3 ; Ciptadi, Gatot^2 ; Setiyawati^3 ; Putri, Ardyah R. I.^2

Central Laboratory of Life Sciences, Brawijaya University, Indonesia^1

Faculty of Animal Science, Brawijaya University, Indonesia^2

Faculty of Mathematics and Science, Biology Departement, Brawijaya University, Indonesia^3

关键词: Cryoprotective agents;    Dimethyl sulfoxide (DMSO);    DMSO;    Fetus;    Fibroblast cells;    Freezing methods;    Goat;    Mr.Frosty;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/546/6/062011/pdf
DOI  :  10.1088/1757-899X/546/6/062011

学科分类：自然科学（综合）

来源: IOP

【 摘 要 】

Dimethyl sulfoxide (DMSO) is one of the cryoprotectants that have been used in cell freezing. Cryoprotective agents are necessary to deal with damage in cells associated with slow freezing. Goat foetuses are obtained from local slaughterhouse in Sukun, Malang, East Java. Using various DMSO concentration that were 0% control, 5%, 10%, and 15%. The cell was free with slow freeze methods using Mr. Frosty in-20°C. After 24 days, the samples were analyzed for their viability, size, and abnormality. The result shows the cell size ranged from 3 μm until 5 μm in all samples, categorized as small cell. In viability is significantly influential on 10% DMSO concentration of 92%, while at 0% concentration is 0%, 5% concentration is 56%, and 15% concentration is 89.67%. The abnormality in DMSO 0% all cells are dead and the tissue isn't developing, in DMSO 5% the abnormality is 3.17%, DMSO 10% is 2.5%, and DMSO 15% 5.67. From the results, we can conclude that the concentration of 10% of DMSO is the best concentration to be used as a freezing method for goat fetus cells.