Effects of dimethyl sulfoxide on asymmetric division and cytokinesis in mouse oocytes

BMC Developmental Biology

Lei Lei¹  Xi Wu¹  Tong Li¹  Na Zhang¹  Yanli Gu¹  Xinghui Shen¹  Dongjie Zhou¹ 

[1] Department of Histology and Embryology, Harbin Medical University, Harbin, China

关键词: Oocyte maturation;    Spindle migration;    Actin cap;    Asymmetric division;    Dimethyl sulfoxide (DMSO);   

Others  :  847194
DOI  :  10.1186/1471-213X-14-28

 received in 2014-02-27, accepted in 2014-06-19,  发布年份 2014

【 摘 要 】

Background

Dimethyl sulfoxide (DMSO) is used extensively as a permeable cryoprotectant and is a common solvent utilized for several water-insoluble substances. DMSO has various biological and pharmacological activities; however, the effect of DMSO on mouse oocyte meiotic maturation remains unknown.

Results

In DMSO-treated oocytes, we observed abnormal MII oocytes that contained large polar bodies, including 2-cell–like MII oocytes, during in vitro maturation. Oocyte polarization did not occur, due to the absence of actin cap formation and spindle migration. These features are among the primary causes of abnormal symmetric division; however, analysis of the mRNA expression levels of genes related to asymmetric division revealed no significant difference in the expression of these factors between the 3% DMSO-treated group and the control group. After each “blastomere” of the 2-cell–like MII stage oocytes was injected by one sperm head respectively, the oocytes still possessed the ability to extrude the second polar body from each “blastomere” and to begin cleavage. However, MII oocytes with large polar bodies developed to the blastocyst stage after intracytoplasmic sperm injection (ICSI). Furthermore, other permeable cryoprotectants, such as ethylene glycol and glycerol, also caused asymmetric division failure.

Conclusion

Permeable cryoprotectants, such as DMSO, ethylene glycol, and glycerol, affect asymmetric division. DMSO disrupts cytokinesis completion by inhibiting cortical reorganization and polarization. Oocytes that undergo symmetric division maintain the ability to begin cleavage after ICSI.

【 授权许可】

   
2014 Zhou et al.; licensee BioMed Central Ltd.

【 图 表 】

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