The kinases PIG-1 and PAR-1 act in redundant pathways to regulate asymmetric division in the EMS blastomere of C. elegans

Developmental biology

Diane G. Morton^2,1¹  Małgorzata J. Liro^1² 

[1] Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA^2;Department of Molecular and Cellular Biology and Graduate Program in Biochemistry, Molecular, Cellular and Developmental Biology, University of California, Davis, CA 95616, USA^1

关键词: pig-1;    par-1;    let‐99;    Spindle orientation;    Asymmetric division;    Endoderm specification;   

DOI  :  10.1016/j.ydbio.2018.08.016

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

The PAR-1 kinase of C. elegans is localized to the posterior of the one-cell embryo and its mutations affect asymmetric spindle placement and partitioning of cytoplasmic components in the first cell cycle. However, par-1 mutations do not cause failure to restrict the anterior PAR polarity complex to the same extent as mutations in the posteriorly localized PAR-2 protein. Further, it has been difficult to examine the role of PAR-1 in subsequent divisions due to the early defects in par-1 mutant embryos. Here we show that the PIG-1 kinase acts redundantly with PAR-1 to restrict the anterior PAR-3 protein for normal polarity in the one-cell embryo. By using a temperature sensitive allele of par-1, which exhibits enhanced lethality when combined with a pig-1 mutation, we have further explored roles for these genes in subsequent divisions. We find that both PIG-1 and PAR-1 regulate spindle orientation in the EMS blastomere of the four-cell stage embryo to ensure that it undergoes an asymmetric division. In this cell, PIG-1 and PAR-1 act in parallel pathways for spindle positioning, PIG-1 in the MES-1/SRC-1 pathway and PAR-1 in the Wnt pathway.

【 授权许可】

CC BY