The Quality of Ettawa Crossbreed Sperm: The Treatment with a Combination of Cryoprotectant in Tris Diluents

International Conference on Life Sciences and Technology

Cahya Prastya Rini, Dwi^1 ; Zaenab, Siti^1 ; Hadi, Samsun^1 ; Jaya Miharja, Fuad^1

Department of Biology Education, Faculty of Teacher Training and Education, University of Muhammadiyah Malang, Jl. Raya Tlogomas No. 246, Malang, Indonesia^1

关键词: Combination treatments;    Cryo-preservation;    DMSO;    Ettawa crossbreed;    Freezing process;    Independent variables;    Negative control;    Observation techniques;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/276/1/012047/pdf
DOI  :  10.1088/1755-1315/276/1/012047

来源: IOP

【 摘 要 】

The formation of ice crystals easily damages the sperm of goats during the freezing process. This study aimed to determine the optimum treatment of cryoprotectant DMSO, glycerol, and a combination of DMSO with glycerol in Tris-egg yolk diluents to maintain the quality of frozen sperm of ettawa breeds. This study used four male goats of which semen was collected twice a week using an artificial vagina. The independent variables in this study were 6% DMSO, 6% glycerol, and a combination of 3% DMSO: 3% glycerol, negative control (without treatment), and positive control (7% glycerol). The sperm quality parameters include motility, viability, and abnormalities. Data collection techniques used observation techniques before and after freezing. The data were tested statistically using one-way ANOVA and Duncan test. The results showed that the combination treatment of 3% DMSO: 3% glycerol produced significant motility, viability, and abnormalities (P