Isolation and preservation of microfungi from Indonesia islands

International Symposium on Bioremediation, Biomaterial, Revegetation, and Conservation

生物科学;自然科学（总论）

Wulandari, N.F.^1

Microbiology Division, Research Centre for Biology, Indonesian Institute of Sciences (LIPI), Cibinong Science Centre, Jl. Raya Jakarta Bogor KM. 46, Cibinong

16119, Indonesia^1

关键词: Cryo-preservation;    Culture collection;    Indonesia;    Long-term preservation;    Micro-fungi;    Potato dextrose agars;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/308/1/012079/pdf
DOI  :  10.1088/1755-1315/308/1/012079

来源: IOP

【 摘 要 】

The research was aimed at utilizing more adequate methods of long-term preservation, cryopreservation (freezing at -80 °C). Samples obtained from three islands were used as sources of samples, i.e., Enggano, Riau, and Sumba. Direct isolation and single spore isolation were conducted to isolate the microfungi. All the cultures were grown on Potato Dextrose Agar (PDA). The growth rate of selected microfungi was calculated every day for a week. The growth rate for selected ten microfungi of each island, approximately 0.1-0.7 cm/day. All the cultures are maintained in InaCC (Indonesian Culture Collections), Cibinong, LIPI.